TY - JOUR T1 - Functional Properties of Two Bombesin-Like Peptide Receptors Revealed by the Analysis of Mice Lacking Neuromedin B Receptor JF - The Journal of Neuroscience JO - J. Neurosci. SP - 948 LP - 954 DO - 10.1523/JNEUROSCI.19-03-00948.1999 VL - 19 IS - 3 AU - Hiroko Ohki-Hamazaki AU - Yasushi Sakai AU - Katsuo Kamata AU - Hiroo Ogura AU - Shigeru Okuyama AU - Kei Watase AU - Kazuyuki Yamada AU - Keiji Wada Y1 - 1999/02/01 UR - http://www.jneurosci.org/content/19/3/948.abstract N2 - The neuromedin B-preferring receptor (NMB-R) is one of the members of the bombesin (BN)-like peptide receptor subfamily in mammals. Previously, we have generated and characterized mice with targeted disruption of the two other BN-like peptide receptors, bombesin receptor subtype-3 (BRS-3) and gastrin-releasing peptide-preferring receptor (GRP-R). Here we describe the generation and analysis of NMB-R-deficient mice to investigate how NMB-R differs from BRS-3 and GRP-R. Compensation for NMB-R deficiency by overexpression of GRP-R and/or BRS-3 was not detected. Although the hypothermic effect of NMB was reduced by 50% in NMB-R-deficient mice, the effect of GRP infusion was comparable to the wild-type mice. In contrast, fundic smooth muscle contraction on stimulation with NMB or GRP was normal in NMB-R-deficient mice. Administration of GRP but not NMB suppressed glucose intake in both normal and NMB-R-deficient mice. These results suggest that the NMB-R has an essential role in thermoregulation, but not for smooth muscle contraction of the fundus or for the suppression of feeding behavior. In addition, the behavioral phenotypes of GRP-R-deficient mice were not observed in NMB-R-deficient mice. These data show that the functions of NMB-R and GRP-R are distinct, with only partial overlap. ER -