RT Journal Article
SR Electronic
T1 Ischemic Tolerance in Murine Cortical Cell Culture: Critical Role for NMDA Receptors
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 1657
OP 1662
DO 10.1523/JNEUROSCI.19-05-01657.1999
VO 19
IS 5
A1 Margaret C. Grabb
A1 Dennis W. Choi
YR 1999
UL http://www.jneurosci.org/content/19/5/1657.abstract
AB Murine cortical cultures containing both neurons and glia (daysin vitro 13–15) were exposed to periods of oxygen–glucose deprivation (5–30 min) too brief to induce neuronal death. Cultures “preconditioned” by sublethal oxygen–glucose deprivation exhibited 30–50% less neuronal death than controls when exposed to a 45–55 min period of oxygen–glucose deprivation 24 hr later. This preconditioning-induced neuroprotection was specific in that neuronal death induced by exposure to excitotoxins or to staurosporine was not attenuated. Neuroprotection was lost if the time between the preconditioning and severe insult were decreased to 7 hr or increased to 72 hr and was blocked if the NMDA antagonist 100 μm3-((d)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid was applied during the preconditioning insult. This was true even if the duration of preconditioning was increased as far as possible (while still remaining sublethal). A similar preconditioning effect was also produced by sublethal exposure to high K+, glutamate, or NMDA but not to kainate ortrans-1-aminocyclopentane-1,3-dicarboxylic acid.