RT Journal Article
SR Electronic
T1 Forced Limb-Use Effects on the Behavioral and Neurochemical Effects of 6-Hydroxydopamine
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 4427
OP 4435
DO 10.1523/JNEUROSCI.21-12-04427.2001
VO 21
IS 12
A1 Jennifer L. Tillerson
A1 Ann D. Cohen
A1 Jennifer Philhower
A1 Gary W. Miller
A1 Michael J. Zigmond
A1 Timothy Schallert
YR 2001
UL http://www.jneurosci.org/content/21/12/4427.abstract
AB Rats with unilateral depletion of striatal dopamine (DA) show marked preferential use of the ipsilateral forelimb. Previous studies have shown that implementation of motor therapy after stroke improves functional outcome (Taub et al., 1999). Thus, we have examined the impact of forced use of the impaired forelimb during or soon after unilateral exposure to the DA neurotoxin 6-hydroxydopamine (6-OHDA). In one group of animals, the nonimpaired forelimb was immobilized using a cast, which forced exclusive use of the impaired limb for the first 7 d after infusion. The animals that received a cast displayed no detectable impairment or asymmetry of limb use, could use the contralateral (impaired) forelimb independently for vertical and lateral weight shifting, and showed no contralateral turning to apomorphine. The behavioral effects were maintained throughout the 60 d of observation. In addition to the behavioral sparing, these animals showed remarkable sparing of striatal DA, its metabolites, and the expression of the vesicular monoamine transporter, suggesting a decrease in the extent of DA neuron degeneration. Behavioral and neurochemical sparing appeared to be complete when the 7 d period of immobilization was initiated immediately after 6-OHDA infusion, only partial sparing was evident when immobilization was initiated 3 d postoperatively, and no sparing was detected when immobilization was initiated 7 d after 6-OHDA treatment. These results suggest that physical therapy may be beneficial in Parkinson's disease.