PT - JOURNAL ARTICLE AU - Mingkui Chen AU - J. Marc Simard TI - Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca<sup>2+</sup> and ATP in Native Reactive Astrocytes from Adult Rat Brain AID - 10.1523/JNEUROSCI.21-17-06512.2001 DP - 2001 Sep 01 TA - The Journal of Neuroscience PG - 6512--6521 VI - 21 IP - 17 4099 - http://www.jneurosci.org/content/21/17/6512.short 4100 - http://www.jneurosci.org/content/21/17/6512.full SO - J. Neurosci.2001 Sep 01; 21 AB - Hypoxia–ischemia and ATP depletion are associated with glial swelling and blebbing, but mechanisms involved in these effects remain incompletely characterized. We examined morphological and electrophysiological responses of freshly isolated native reactive astrocytes (NRAs) after exposure to NaN3, which depletes cellular ATP. Here we report that NaN3 caused profound and sustained depolarization attributable to activation of a novel 35 pS Ca2+-activated, [ATP]i-sensitive nonselective cation (NCCa-ATP) channel, found in &gt;90% of excised membrane patches. The channel was impermeable to Cl−, was nearly equally permeable to monovalent cations, with permeabilities relative to K+ being PCs+/PK+(1.06) ≈ PNa+/PK+(1.04) ≈ PRb+/PK+(1.02) ≈ PLi+/PK+(0.96), and was essentially impermeable to Ca2+ and Mg2+(PCa2+/PK+≈ PMg2+/PK+&lt; 0.001), with intracellular Mg2+ (100 μm to 1 mm) causing inward rectification. Pore radius, estimated by fitting relative permeabilities of organic cations to the Renkin equation, was 0.41 nm. This channel exhibited significantly different properties compared with previously reported NCCa-ATP channels, including different sensitivity to block by various adenine nucleotides (EC50of 0.79 μm for [ATP]i, with no block by AMP or ADP), and activation by submicromolar [Ca]i. The apparent dissociation constant for Ca2+ was voltage dependent (0.12, 0.31, and 1.5 μm at −40, −80, and −120 mV, respectively), with a Hill coefficient of 1.5. Channel opening by [ATP]i depletion was accompanied by and appeared to precede blebbing of the cell membrane, suggesting participation of this channel in cation flux involved in cell swelling. We conclude that NRAs from adult rat brain express a 35 pS NCCa-ATP channel that may play an important role in the pathogenesis of brain swelling.