PT  - JOURNAL ARTICLE
AU  - Li-Lian Yuan
AU  - J. Paige Adams
AU  - Michael Swank
AU  - J. David Sweatt
AU  - Daniel Johnston
TI  - Protein Kinase Modulation of Dendritic K<sup>+</sup> Channels in Hippocampus Involves a Mitogen-Activated Protein Kinase Pathway
AID  - 10.1523/JNEUROSCI.22-12-04860.2002
DP  - 2002 Jun 15
TA  - The Journal of Neuroscience
PG  - 4860--4868
VI  - 22
IP  - 12
4099  - http://www.jneurosci.org/content/22/12/4860.short
4100  - http://www.jneurosci.org/content/22/12/4860.full
SO  - J. Neurosci.2002 Jun 15; 22
AB  - We investigated mitogen-activated protein kinase (MAPK) modulation of dendritic, A-type K+ channels in CA1 pyramidal neurons in the hippocampus. Activation of cAMP-dependent protein kinase A (PKA) and protein kinase C (PKC) leads to an increase in the amplitude of backpropagating action potentials in distal dendrites through downregulation of transient K+ channels in CA1 pyramidal neurons in the hippocampus. We show here that both of these signaling pathways converge on extracellular-regulated kinases (ERK)-specific MAPK in mediating this reduction in dendritic K+ current, which is confirmed, in parallel, by biochemical assays using phosphospecific antibodies against the ppERK and pKv4.2. Furthermore, immunostaining indicates dendritic localization of ppERK and pKv4.2. Taken together, these results demonstrate that dendritic, A-type K+ channels are dually regulated by PKA and PKC through a common downstream pathway involving MAPK, and the modulation of these K+channels may be accounted for by the phosphorylation of Kv4.2 subunits.