RT Journal Article
SR Electronic
T1 A Chondroitin Sulfate Proteoglycan PTPζ/RPTPβ Regulates the Morphogenesis of Purkinje Cell Dendrites in the Developing Cerebellum
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 2804
OP 2814
DO 10.1523/JNEUROSCI.23-07-02804.2003
VO 23
IS 7
A1 Masahiko Tanaka
A1 Nobuaki Maeda
A1 Masaharu Noda
A1 Tohru Marunouchi
YR 2003
UL http://www.jneurosci.org/content/23/7/2804.abstract
AB PTPζ/RPTPβ, a receptor-type protein tyrosine phosphatase synthesized as a chondroitin sulfate (CS) proteoglycan, uses a heparin-binding growth factor pleiotrophin (PTN) as a ligand, in which the CS portion plays an essential role in ligand binding. Using an organotypic slice culture system, we tested the hypothesis that PTN-PTPζ signaling is involved in the morphogenesis of Purkinje cell dendrites. An aberrant morphology of Purkinje cell dendrites such as multiple and disoriented primary dendrites was induced in slice cultures by (1) addition of a polyclonal antibody against the extracellular domain of PTPζ, (2) inhibition of protein tyrosine phosphatase activity, (3) enzymatic removal of the CS chains, (4) addition of exogenous CS chains, and (5) addition of exogenous PTN, all of which disturb PTN-PTPζ signaling. These treatments also reduced the immunoreactivity to GLAST, a glial glutamate transporter, on Bergmann glial processes. Furthermore, a glutamate transporter inhibitor also induced the abnormal morphogenesis of Purkinje cell dendrites. Altogether, these findings suggest that PTN-PTPζ signaling regulates the morphogenesis of Purkinje cell dendrites and that the mechanisms underlying that regulation involve the GLAST activity in Bergmann glial processes.