PT - JOURNAL ARTICLE AU - Christopher A. Reid AU - Don B. Dixon AU - Michiko Takahashi AU - Tim V. P. Bliss AU - Alan Fine TI - Optical Quantal Analysis Indicates That Long-Term Potentiation at Single Hippocampal Mossy Fiber Synapses Is Expressed through Increased Release Probability, Recruitment of New Release Sites, and Activation of Silent Synapses AID - 10.1523/JNEUROSCI.3567-03.2004 DP - 2004 Apr 07 TA - The Journal of Neuroscience PG - 3618--3626 VI - 24 IP - 14 4099 - http://www.jneurosci.org/content/24/14/3618.short 4100 - http://www.jneurosci.org/content/24/14/3618.full SO - J. Neurosci.2004 Apr 07; 24 AB - It is generally believed that long-term potentiation (LTP) at hippocampal mossy fiber synapses between dentate granule and CA3 pyramidal cells is expressed through presynaptic mechanisms leading to an increase in quantal content. The source of this increase has remained undefined but could include enhanced probability of transmitter release at existing functional release sites or increases in the number of active release sites. We performed optical quantal analyses of transmission at individual mossy fiber synapses in cultured hippocampal slices, using confocal microscopy and intracellular fluorescent Ca2+ indicators. Our results indicate that LTP is expressed at functional synapses by both increased probability of transmitter release and recruitment of new release sites, including the activation of previously silent synapses here visualized for the first time.