TY - JOUR T1 - GABA Uptake via GABA Transporter-1 Modulates GABAergic Transmission in the Immature Hippocampus JF - The Journal of Neuroscience JO - J. Neurosci. SP - 5877 LP - 5880 DO - 10.1523/JNEUROSCI.1287-04.2004 VL - 24 IS - 26 AU - Sampsa Sipilä AU - Kristiina Huttu AU - Juha Voipio AU - Kai Kaila Y1 - 2004/06/30 UR - http://www.jneurosci.org/content/24/26/5877.abstract N2 - GABA uptake limits GABA actions during synaptic responses when the density of active release sites is high or multiple axons are synchronously activated. GABA transporter-1 (GAT-1) is the main neuronal GABA transporter subtype and is already expressed in the early postnatal rat hippocampus. However, previous studies have demonstrated little functional role for the transporter during this developmental period. We used whole-cell voltage-clamp and field-potential recordings in hippocampal slices of neonatal rats (postnatal day 4-5) to study whether GAT-1 plays a role in GABAergic transmission during spontaneous population oscillations, which are seen as “giant depolarizing potentials” (GDPs) in intracellular recordings. We show that the GDP-associated GABAergic current observed in CA3 pyramidal neurons is strongly enhanced by the GAT-1-specific blocker NO-711 (1-[2-[[(diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride). Our results indicate a novel role for GAT-1 in the control of endogenous activity of the immature hippocampus. ER -