RT Journal Article SR Electronic T1 GABA Uptake via GABA Transporter-1 Modulates GABAergic Transmission in the Immature Hippocampus JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 5877 OP 5880 DO 10.1523/JNEUROSCI.1287-04.2004 VO 24 IS 26 A1 Sampsa Sipilä A1 Kristiina Huttu A1 Juha Voipio A1 Kai Kaila YR 2004 UL http://www.jneurosci.org/content/24/26/5877.abstract AB GABA uptake limits GABA actions during synaptic responses when the density of active release sites is high or multiple axons are synchronously activated. GABA transporter-1 (GAT-1) is the main neuronal GABA transporter subtype and is already expressed in the early postnatal rat hippocampus. However, previous studies have demonstrated little functional role for the transporter during this developmental period. We used whole-cell voltage-clamp and field-potential recordings in hippocampal slices of neonatal rats (postnatal day 4-5) to study whether GAT-1 plays a role in GABAergic transmission during spontaneous population oscillations, which are seen as “giant depolarizing potentials” (GDPs) in intracellular recordings. We show that the GDP-associated GABAergic current observed in CA3 pyramidal neurons is strongly enhanced by the GAT-1-specific blocker NO-711 (1-[2-[[(diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride). Our results indicate a novel role for GAT-1 in the control of endogenous activity of the immature hippocampus.