PT  - JOURNAL ARTICLE
AU  - Hui-Yun Cheng
AU  - David F. Clayton
TI  - Activation and Habituation of Extracellular Signal-Regulated Kinase Phosphorylation in Zebra Finch Auditory Forebrain during Song Presentation
AID  - 10.1523/JNEUROSCI.1405-04.2004
DP  - 2004 Aug 25
TA  - The Journal of Neuroscience
PG  - 7503--7513
VI  - 24
IP  - 34
4099  - http://www.jneurosci.org/content/24/34/7503.short
4100  - http://www.jneurosci.org/content/24/34/7503.full
SO  - J. Neurosci.2004 Aug 25; 24
AB  - The sound of tape-recorded birdsong triggers a set of behavioral and physiological responses in zebra finches, including transcriptional activation of the zenk gene in the auditory forebrain. Song repetition leads to the stimulus-specific habituation of these responses. To gain insight into the mechanisms that couple auditory experience to gene regulation, we monitored the phosphorylation of the zebra finch extracellular signal-regulated kinase (ERK) protein by immunoblotting. Initial presentations of novel song (but not tones or noise) resulted in a rapid increase in ERK phosphorylation, followed by a return to basal levels within 5 min. This response was localized to the auditory forebrain where the zenk gene is activated. Sustained repetition of one song caused a selective habituation of the ERK response: a different song triggered another cycle of ERK phosphorylation without altering the habituated response to the first. To test directly for a role of ERK in experience-dependent zenk gene regulation, we infused an inhibitor of mitogen-activated and extracellular-regulated protein kinase kinase (MEK-1; the enzyme responsible for ERK activation) unilaterally into one auditory lobule just before song stimulation. The song-induced increase in zenk mRNA was blocked on the side of the injection, but not on the contralateral (uninfused) side. These results show that ERK phosphorylation is necessary for the initiation of the zenk gene response to novel song and identify ERK as a plausible site of signal integration underlying the selective habituation of genomic responses to a repeated song.