PT - JOURNAL ARTICLE AU - Bénédicte Franco AU - Laurent Bogdanik AU - Yves Bobinnec AU - Alain Debec AU - Joël Bockaert AU - Marie-Laure Parmentier AU - Yves Grau TI - Shaggy, the Homolog of Glycogen Synthase Kinase 3, Controls Neuromuscular Junction Growth in <em>Drosophila</em> AID - 10.1523/JNEUROSCI.1580-04.2004 DP - 2004 Jul 21 TA - The Journal of Neuroscience PG - 6573--6577 VI - 24 IP - 29 4099 - http://www.jneurosci.org/content/24/29/6573.short 4100 - http://www.jneurosci.org/content/24/29/6573.full SO - J. Neurosci.2004 Jul 21; 24 AB - A protein-trap screen using the Drosophila neuromuscular junction (NMJ) as a model synapse was performed to identify genes that control synaptic structure or plasticity. We found that Shaggy (Sgg), the Drosophila homolog of the mammalian glycogen synthase kinases 3 α and β, two serine-threonine kinases, was concentrated at this synapse. Using various combinations of mutant alleles of shaggy, we found that Shaggy negatively controlled the NMJ growth. Moreover, tissue-specific expression of a dominant-negative Sgg indicated that this kinase is required in the motoneuron, but not in the muscle, to control NMJ growth. Finally, we show that Sgg controlled the microtubule cytoskeleton dynamics in the motoneuron and that Futsch, a microtubule-associated protein, was required for Shaggy function on synaptic growth.