PT  - JOURNAL ARTICLE
AU  - Carol J. Milligan
AU  - Noel J. Buckley
AU  - Maurice Garret
AU  - Jim Deuchars
AU  - Susan A. Deuchars
TI  - Evidence for Inhibition Mediated by Coassembly of GABA<sub>A</sub> and GABA<sub>C</sub> Receptor Subunits in Native Central Neurons
AID  - 10.1523/JNEUROSCI.1979-04.2004
DP  - 2004 Aug 18
TA  - The Journal of Neuroscience
PG  - 7241--7250
VI  - 24
IP  - 33
4099  - http://www.jneurosci.org/content/24/33/7241.short
4100  - http://www.jneurosci.org/content/24/33/7241.full
SO  - J. Neurosci.2004 Aug 18; 24
AB  - Fast inhibition in the nervous system is commonly mediated by GABAA receptors comprised of 2α/2β/1γ subunits. In contrast, GABAC receptors containing onlyρ subunits (ρ1-ρ3) have been predominantly detected in the retina. However, here using reverse transcription-PCR and in situ hybridization we show that mRNA encoding the ρ1 subunit is highly expressed in brainstem neurons. Immunohistochemistry localized the ρ1 subunit to neurons at light and electron microscopic levels, where it was detected at synaptic junctions. Application of the GABAC receptor agonist cis-4-aminocrotonic acid (100-800 μM) requires the ρ1 subunit to elicit responses, which surprisingly are blocked independently by antagonists to GABAA (bicuculline, 10 μM) and GABAC [(1,2,5,6-tetrahydropyridin-4-yl)methylphosphinic acid (TPMPA); 40-160 μM] receptors. Responses to GABAC agonists were also enhanced by the GABAA receptor modulator pentobarbitone (300 μM). Spontaneous and evoked IPSPs were reduced in amplitude but never abolished by TPMPA, but were completely blocked by bicuculline. We therefore tested the hypothesis that GABAA and GABAC subunits formed a heteromeric receptor. Immunohistochemistry indicated that ρ1 and α1 subunits were colocalized at light and electron microscopic levels. Electrophysiology revealed that responses to GABAC receptor agonists were enhanced by the GABAA receptor modulator zolpidem (500 nm), which acts on the α1 subunit when the γ2 subunit is also present. Finally, coimmunoprecipitation indicated that the ρ1 subunit formed complexes that also containedα1 and γ2 subunits. Taken together these separate lines of evidence suggest that the effects of GABA in central neurons can be mediated by heteromeric complexes of GABAA and GABAC receptor subunits.