TY - JOUR T1 - Two Interdependent TRPV Channel Subunits, Inactive and Nanchung, Mediate Hearing in <em>Drosophila</em> JF - The Journal of Neuroscience JO - J. Neurosci. SP - 9059 LP - 9066 DO - 10.1523/JNEUROSCI.1645-04.2004 VL - 24 IS - 41 AU - Zhefeng Gong AU - Wonseok Son AU - Yun Doo Chung AU - Janghwan Kim AU - Dong Wook Shin AU - Colleen A. McClung AU - Yong Lee AU - Hye Won Lee AU - Deok-Jin Chang AU - Bong-Kiun Kaang AU - Hawon Cho AU - Uhtaek Oh AU - Jay Hirsh AU - Maurice J. Kernan AU - Changsoo Kim Y1 - 2004/10/13 UR - http://www.jneurosci.org/content/24/41/9059.abstract N2 - Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons in Johnston's organ, the antennal chordotonal organ. We previously found that a Drosophila protein in the vanilloid receptor subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to the chordotonal cilia and required to generate sound-evoked potentials (Kim et al., 2003). Here, we show that the only other Drosophila TRPV protein is mutated in the behavioral mutant inactive (iav). The IAV protein forms a hypotonically activated channel when expressed in cultured cells; in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing. IAV and NAN are each undetectable in cilia of mutants lacking the other protein, indicating that they both contribute to a heteromultimeric transduction channel in vivo. A functional green fluorescence protein-IAV fusion protein shows that the channel is restricted to the proximal cilium, constraining models for channel activation. ER -