RT Journal Article
SR Electronic
T1 Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 10999
OP 11009
DO 10.1523/JNEUROSCI.3934-04.2004
VO 24
IS 49
A1 Yi Zuo
A1 Jane L. Lubischer
A1 Hyuno Kang
A1 Le Tian
A1 Michelle Mikesh
A1 Alexander Marks
A1 Virginia L. Scofield
A1 Shan Maika
A1 Craig Newman
A1 Paul Krieg
A1 Wesley J. Thompson
YR 2004
UL http://www.jneurosci.org/content/24/49/10999.abstract
AB To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury.