TY - JOUR T1 - Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination JF - The Journal of Neuroscience JO - J. Neurosci. SP - 10999 LP - 11009 DO - 10.1523/JNEUROSCI.3934-04.2004 VL - 24 IS - 49 AU - Yi Zuo AU - Jane L. Lubischer AU - Hyuno Kang AU - Le Tian AU - Michelle Mikesh AU - Alexander Marks AU - Virginia L. Scofield AU - Shan Maika AU - Craig Newman AU - Paul Krieg AU - Wesley J. Thompson Y1 - 2004/12/08 UR - http://www.jneurosci.org/content/24/49/10999.abstract N2 - To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury. ER -