@article {Ratzliff2259, author = {Anna d. H. Ratzliff and Allyson L. Howard and Vijayalakshmi Santhakumar and Imola Osapay and Ivan Soltesz}, title = {Rapid Deletion of Mossy Cells Does Not Result in a Hyperexcitable Dentate Gyrus: Implications for Epileptogenesis}, volume = {24}, number = {9}, pages = {2259--2269}, year = {2004}, doi = {10.1523/JNEUROSCI.5191-03.2004}, publisher = {Society for Neuroscience}, abstract = {Loss of cells from the hilus of the dentate gyrus is a major histological hallmark of human temporal lobe epilepsy. Hilar mossy cells, in particular, are thought to show dramatic numerical reductions in pathological conditions, and one prominent theory of epileptogenesis is based on the assumption that mossy cell loss directly results in granule cell hyperexcitability. However, whether it is the disappearance of hilar mossy cells from the dentate gyrus circuitry after various insults or the subsequent synaptic-cellular alterations (e.g., reactive axonal sprouting) that lead to dentate hyperexcitability has not been rigorously tested, because of the lack of available techniques to rapidly remove specific classes of nonprincipal cells from neuronal networks. We developed a fast, cell-specific ablation technique that allowed the targeted lesioning of either mossy cells or GABAergic interneurons in horizontal as well as axial (longitudinal) slices of the hippocampus. The results demonstrate that mossy cell deletion consistently decreased the excitability of granule cells to perforant path stimulation both within and outside of the lamella where the mossy cell ablation took place. In contrast, ablation of interneurons caused the expected increase in excitability, and control aspirations of the hilar neuropil or of interneurons in the presence of GABA receptor blockers caused no alteration in granule cell excitability. These data do not support the hypothesis that loss of mossy cells from the dentate hilus after seizures or traumatic brain injury directly results in hyperexcitability.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/24/9/2259}, eprint = {https://www.jneurosci.org/content/24/9/2259.full.pdf}, journal = {Journal of Neuroscience} }