PT  - JOURNAL ARTICLE
AU  - Masahito Yamagata
AU  - Joshua R. Sanes
TI  - Versican in the Developing Brain: Lamina-Specific Expression in Interneuronal Subsets and Role in Presynaptic Maturation
AID  - 10.1523/JNEUROSCI.1976-05.2005
DP  - 2005 Sep 14
TA  - The Journal of Neuroscience
PG  - 8457--8467
VI  - 25
IP  - 37
4099  - http://www.jneurosci.org/content/25/37/8457.short
4100  - http://www.jneurosci.org/content/25/37/8457.full
SO  - J. Neurosci.2005 Sep 14; 25
AB  - Chondroitin sulfate proteoglycans (CSPGs) of the extracellular matrix help stabilize synaptic connections in the postnatal brain and impede regeneration after injury. Here, we show that a CSPG of the lectican family, versican, also promotes presynaptic maturation in the developing brain. In the embryonic chick optic tectum, versican is expressed selectively by subsets of interneurons confined to the retinorecipient laminae, in which retinal axons arborize and form synapses. It is a major receptor for the Vicia villosa B4 lectin (VVA), shown previously to inhibit invasion of the retinorecipient lamina by retinal axons (Inoue and Sanes, 1997). In vitro, versican promotes enlargement of presynaptic varicosities in retinal axons. Depletion of versican in ovo, by RNA interference, results in retinal arbors with smaller than normal varicosities. We propose that versican provides a lamina-specific cue for presynaptic maturation and discuss the related but distinct effects of versican depletion and VVA blockade.