RT Journal Article
SR Electronic
T1 Activity-Dependent Movements of Postsynaptic Scaffolds at Inhibitory Synapses
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 4586
OP 4595
DO 10.1523/JNEUROSCI.5123-05.2006
VO 26
IS 17
A1 Cyril Hanus
A1 Marie-Virginie Ehrensperger
A1 Antoine Triller
YR 2006
UL http://www.jneurosci.org/content/26/17/4586.abstract
AB Dendritic spines show an activity-dependent cytoskeleton-based remodeling coupled with variations in receptor number and the functional properties of excitatory synapses. In this study, we analyzed the dynamics of gephyrin containing inhibitory postsynaptic scaffolds imaging a Venus::gephyrin (VeGe) chimera in dissociated spinal cord neurons. We provide evidence that the postsynaptic scaffolds at mature synapses display a submicrometric rapid lateral motion and are continuously moving on the dendritic shaft. This dynamic behavior is calcium dependent and is controlled by the cytoskeleton. Minute rearrangement within the gephyrin scaffold as well as the scaffold lateral displacements are F-actin dependent. The lateral movements are counteracted by microtubules. Moreover, the action of the potassium channel blocker 4-aminopyridine and receptor antagonists indicate that the dynamics of postsynaptic gephyrin scaffolds are controlled by synaptic activity.