PT  - JOURNAL ARTICLE
AU  - Nicolas Gervasi
AU  - Régine Hepp
AU  - Ludovic Tricoire
AU  - Jin Zhang
AU  - Bertrand Lambolez
AU  - Danièle Paupardin-Tritsch
AU  - Pierre Vincent
TI  - Dynamics of Protein Kinase A Signaling at the Membrane, in the Cytosol, and in the Nucleus of Neurons in Mouse Brain Slices
AID  - 10.1523/JNEUROSCI.5352-06.2007
DP  - 2007 Mar 14
TA  - The Journal of Neuroscience
PG  - 2744--2750
VI  - 27
IP  - 11
4099  - http://www.jneurosci.org/content/27/11/2744.short
4100  - http://www.jneurosci.org/content/27/11/2744.full
SO  - J. Neurosci.2007 Mar 14; 27
AB  - The cAMP-dependent protein kinase A (PKA) plays a ubiquitous role in the regulation of neuronal activity, but the dynamics of its activation have been difficult to investigate. We used the genetically encoded fluorescent probe AKAR2 to record PKA activation in the cytosol and the nucleus of neurons in mouse brain slice preparations, whereas the potassium current underlying the slow afterhyperpolarization potential (sAHP) in thalamic intralaminar neurons was used to monitor PKA activation at the membrane. Adenylyl cyclase was stimulated either directly using forskolin or via activation of 5-HT7 receptors. Both stimulations produced a maximal effect on sAHP, whereas in the cytosol, the amplitude of the 5-HT7 receptor-mediated response was half of that after direct adenylyl cyclase stimulation with forskolin. 5-HT7-mediated PKA responses were obtained in 30 s at the membrane, in 2.5 min in the cytosol, and in 13 min in the nucleus. Our results show in morphologically intact mammalian neurons the potential physiological relevance of PKA signal integration at the subcellular level: neuromodulators produce fast and powerful effects on membrane excitability, consistent with a highly efficient functional coupling between adenylyl cyclases, PKA, and target channels. Phosphorylation in the cytosol is slower and of graded amplitude, showing a differential integration of the PKA signal between the membrane and the cytosol. The nucleus integrates these cytosolic signals over periods of tens of minutes, consistent with passive diffusion of the free catalytic subunit of PKA into the nucleus, eventually resulting in a graded modulation of gene expression.