TY - JOUR T1 - Gene Expression Profiling in Postmortem Prefrontal Cortex of Major Depressive Disorder JF - The Journal of Neuroscience JO - J. Neurosci. SP - 13329 LP - 13340 DO - 10.1523/JNEUROSCI.4083-07.2007 VL - 27 IS - 48 AU - Hyo Jung Kang AU - David H. Adams AU - Arthur Simen AU - Birgitte B. Simen AU - Grazyna Rajkowska AU - Craig A. Stockmeier AU - James C. Overholser AU - Herbert Y. Meltzer AU - George J. Jurjus AU - Lisa C. Konick AU - Samuel S. Newton AU - Ronald S. Duman Y1 - 2007/11/28 UR - http://www.jneurosci.org/content/27/48/13329.abstract N2 - Investigations of the molecular mechanisms underlying major depressive disorder (MDD) have been hampered by the complexity of brain tissue and sensitivity of gene expression profiling approaches. To address these issues, we used discrete microdissections of postmortem dorsolateral prefrontal cortex (DLPFC) (area 9) and an oligonucleotide (60mer) microarray hybridization procedure that increases sensitivity without RNA amplification. Mixed-effects statistical methods were used to rigorously control for medication usage in the subset of medicated depressed subjects. These analyses yielded a rich profile of dysregulated genes. Two of the most highly dysregulated genes of interest were stresscopin, a neuropeptide involved in stress responses, and Forkhead box D3 (FOXD3), a transcription factor. Secondary cell-based analysis demonstrated that stresscopin and FoxD3 are increased in neurons of DLPFC gray matter of MDD subjects. These findings identify abnormal gene expression in a discrete region of MDD subjects and contribute to further elucidation of the molecular alterations of this complex mood disorder. ER -