TY - JOUR T1 - Gβ5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells JF - The Journal of Neuroscience JO - J. Neurosci. SP - 14199 LP - 14204 DO - 10.1523/JNEUROSCI.4934-07.2007 VL - 27 IS - 51 AU - Anjali Rao AU - Rebecca Dallman AU - Scott Henderson AU - Ching-Kang Chen Y1 - 2007/12/19 UR - http://www.jneurosci.org/content/27/51/14199.abstract N2 - Gβ5 exists as two splice variants, Gβ5-S and Gβ5-L, which interact with and stabilize the R7 members of the regulators of G-protein signaling (RGSs): RGS6, RGS7, RGS9, and RGS11. Although the role of Gβ5-L and RGS9-1 is established in photoreceptors, the physiological functions of Gβ5-S and other R7 RGS proteins remain unclear. We found that the electroretinogram of Gβ5−/− mice lacks the b-wave component and that Gβ5-S and RGS11 colocalize with Goα at the tips of the ON-bipolar cell dendrites. Unexpectedly, we found a significant reduction in the number of synaptic triads in the outer plexiform layer (OPL) of the Gβ5−/− mice, which is evident at postnatal day 14. Transgenic expression of Gβ5-L in rods failed to rescue the b-wave or the OPL defects. These results indicate that Gβ5-S is indispensable for OPL integrity and normal light responses of the retina. ER -