@article {Delaunay2551, author = {Delphine Delaunay and Katharina Heydon and Ana Cumano and Markus H. Schwab and Jean-L{\'e}on Thomas and Ueli Suter and Klaus-Armin Nave and Bernard Zalc and Nathalie Spassky}, title = {Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells}, volume = {28}, number = {10}, pages = {2551--2562}, year = {2008}, doi = {10.1523/JNEUROSCI.5497-07.2008}, publisher = {Society for Neuroscience}, abstract = {The question of how neurons and glial cells are generated during the development of the CNS has over time led to two alternative models: either neuroepithelial cells are capable of giving rise to neurons first and to glial cells at a later stage (switching model), or they are intrinsically committed to generate one or the other (segregating model). Using the developing diencephalon as a model and by selecting a subpopulation of ventricular cells, we analyzed both in vitro, using clonal analysis, and in vivo, using inducible Cre/loxP fate mapping, the fate of neuroepithelial and radial glial cells generated at different time points during embryonic development. We found that, during neurogenic periods [embryonic day 9.5 (E9.5) to 12.5], proteolipid protein (plp)-expressing cells were lineage-restricted neuronal precursors, but later in embryogenesis, during gliogenic periods (E13.5 to early postnatal), plp-expressing cells were lineage-restricted glial precursors. In addition, we show that glial cells forming at E13.5 arise from a new pool of neuroepithelial progenitors distinct from neuronal progenitors cells, which lends support to the segregating model.}, issn = {0270-6474}, URL = {https://www.jneurosci.org/content/28/10/2551}, eprint = {https://www.jneurosci.org/content/28/10/2551.full.pdf}, journal = {Journal of Neuroscience} }