PT  - JOURNAL ARTICLE
AU  - Delphine Delaunay
AU  - Katharina Heydon
AU  - Ana Cumano
AU  - Markus H. Schwab
AU  - Jean-Léon Thomas
AU  - Ueli Suter
AU  - Klaus-Armin Nave
AU  - Bernard Zalc
AU  - Nathalie Spassky
TI  - Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells
AID  - 10.1523/JNEUROSCI.5497-07.2008
DP  - 2008 Mar 05
TA  - The Journal of Neuroscience
PG  - 2551--2562
VI  - 28
IP  - 10
4099  - http://www.jneurosci.org/content/28/10/2551.short
4100  - http://www.jneurosci.org/content/28/10/2551.full
SO  - J. Neurosci.2008 Mar 05; 28
AB  - The question of how neurons and glial cells are generated during the development of the CNS has over time led to two alternative models: either neuroepithelial cells are capable of giving rise to neurons first and to glial cells at a later stage (switching model), or they are intrinsically committed to generate one or the other (segregating model). Using the developing diencephalon as a model and by selecting a subpopulation of ventricular cells, we analyzed both in vitro, using clonal analysis, and in vivo, using inducible Cre/loxP fate mapping, the fate of neuroepithelial and radial glial cells generated at different time points during embryonic development. We found that, during neurogenic periods [embryonic day 9.5 (E9.5) to 12.5], proteolipid protein (plp)-expressing cells were lineage-restricted neuronal precursors, but later in embryogenesis, during gliogenic periods (E13.5 to early postnatal), plp-expressing cells were lineage-restricted glial precursors. In addition, we show that glial cells forming at E13.5 arise from a new pool of neuroepithelial progenitors distinct from neuronal progenitors cells, which lends support to the segregating model.