TY - JOUR T1 - α-Ca<sup>2+</sup>/Calmodulin-Dependent Protein Kinase II Contributes to the Developmental Programming of Anxiety in Serotonin Receptor 1A Knock-Out Mice JF - The Journal of Neuroscience JO - J. Neurosci. SP - 6250 LP - 6257 DO - 10.1523/JNEUROSCI.5219-07.2008 VL - 28 IS - 24 AU - Luisa Lo Iacono AU - Cornelius Gross Y1 - 2008/06/11 UR - http://www.jneurosci.org/content/28/24/6250.abstract N2 - Mice lacking the serotonin receptor 1A [Htr1aknock-out (Htr1aKO)] display increased innate and conditioned anxiety-related behavior. Expression of the receptor in the mouse forebrain during development is sufficient to restore normal anxiety-related behavior to knock-out mice, demonstrating a role for serotonin in the developmental programming of anxiety circuits. However, the precise developmental period as well as the signaling pathways and neural substrates involved in this phenomenon are unknown. Here, we show that pharmacological blockade of the receptor from postnatal day 13 (P13)–P34 is sufficient to reproduce the knock-out phenotype in adulthood, thus defining a role for serotonin in the maturation and refinement of anxiety circuits during a limited postnatal period. Furthermore, we identify increases in the phosphorylation of α-Ca2+/calmodulin-dependent protein kinase II (αCaMKII) at threonine 286 in the hippocampus of young Htr1aKO mice under anxiety-provoking conditions. Increases in αCaMKII phosphorylation were most pronounced in the CA1 region of the hippocampus and were localized to the extrasynaptic compartment, consistent with a tissue-specific effect of the receptor. No changes in αCaMKII phosphorylation were found in adult knock-out mice, suggesting a transient role of αCaMKII as a downstream target of the receptor. Finally, the anxiety phenotype was abolished when knock-out mice were crossed to mice in which αCaMKII phosphorylation was compromised by the heterozygous mutation of threonine 286 into alanine. These findings suggest that modulation of αCaMKII function by serotonin during a restricted postnatal period contributes to the developmental programming of anxiety-related behavior. ER -