TY - JOUR T1 - Postsynaptic Action of Brain-Derived Neurotrophic Factor Attenuates α7 Nicotinic Acetylcholine Receptor-Mediated Responses in Hippocampal Interneurons JF - The Journal of Neuroscience JO - J. Neurosci. SP - 5611 LP - 5618 DO - 10.1523/JNEUROSCI.5378-07.2008 VL - 28 IS - 21 AU - Catarina C. Fernandes AU - António Pinto-Duarte AU - Joaquim Alexandre Ribeiro AU - Ana M. Sebastião Y1 - 2008/05/21 UR - http://www.jneurosci.org/content/28/21/5611.abstract N2 - Nicotinic mechanisms acting on the hippocampus influence attention, learning, and memory and constitute a significant therapeutic target for many neurodegenerative, neurological, and psychiatric disorders. Here, we report that brain-derived neurotrophic factor (BDNF) (1–100 ng/ml), a member of the neurotrophin gene family, rapidly decreases α7 nicotinic acetylcholine receptor responses in interneurons of the hippocampal CA1 stratum radiatum. Such effect is dependent on the activation of the TrkB receptor and involves the actin cytoskeleton; noteworthy, it is compromised when the extracellular levels of the endogenous neuromodulator adenosine are reduced with adenosine deaminase (1 U/ml) or when adenosine A2A receptors are blocked with SCH 58261 (2-(2-furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine) (100 nm). The intracellular application of U73122 (1-[6[[(17β)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione) (5 μm), a broad-spectrum inhibitor of phospholipase C, or GF 109203X (bisindolylmaleimide I) (2 μm), a general inhibitor of protein kinase C isoforms, blocks BDNF-induced inhibition of α7 nicotinic acetylcholine receptor function. Moreover, in conditions of simultaneous intracellular dialysis of the fast Ca2+ chelator BAPTA (10 mm) and removal of extracellular Ca2+ ions, the inhibitory action of BDNF is further prevented. The present findings disclose a novel target for rapid actions of BDNF that might play important roles on synaptic transmission and plasticity in the brain. ER -