RT Journal Article
SR Electronic
T1 SCLIP Is Crucial for the Formation and Development of the Purkinje Cell Dendritic Arbor
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 7387
OP 7398
DO 10.1523/JNEUROSCI.1942-08.2008
VO 28
IS 29
A1 Fabienne E. Poulain
A1 Stéphanie Chauvin
A1 Rosine Wehrlé
A1 Mathieu Desclaux
A1 Jacques Mallet
A1 Guilan Vodjdani
A1 Isabelle Dusart
A1 André Sobel
YR 2008
UL http://www.jneurosci.org/content/28/29/7387.abstract
AB Cerebellar Purkinje cells elaborate one of the most complex dendritic arbors among neurons to integrate the numerous signals they receive from the cerebellum circuitry. Their dendritic differentiation undergoes successive, tightly regulated phases of development involving both regressive and growth events. Although many players regulating the late phases of Purkinje cell dendritogenesis have been identified, intracellular factors controlling earlier phases of dendritic development remain mostly unknown. In this study, we explored the biological properties and functions of SCLIP, a protein of the stathmin family, in Purkinje cell dendritic differentiation and cerebellum development. Unlike the other stathmins, SCLIP is strongly expressed in Purkinje cells during cerebellar development and accumulates in their dendritic processes at a critical period of their formation and outgrowth. To reveal SCLIP functions, we developed a lentiviral-mediated approach on cerebellar organotypic cultures to inhibit or increase its expression in Purkinje cells in their tissue environment. Depletion of SCLIP promoted retraction of the Purkinje cell primitive process and then prevented the formation of new dendrites at early stages of postnatal development. It also prevented their elongation and branching at later phases of differentiation. Conversely, SCLIP overexpression promoted dendritic branching and development. Together, our results demonstrate for the first time that SCLIP is crucial for both the formation and proper development of Purkinje cell dendritic arbors. SCLIP appears thus as a novel and specific factor that controls the early phases of Purkinje cell dendritic differentiation during cerebellum development.