RT Journal Article
SR Electronic
T1 Specific Glial Populations Regulate Hippocampal Morphogenesis
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 12328
OP 12340
DO 10.1523/JNEUROSCI.4000-08.2008
VO 28
IS 47
A1 Guy Barry
A1 Michael Piper
A1 Charlotta Lindwall
A1 Randal Moldrich
A1 Sharon Mason
A1 Erica Little
A1 Anindita Sarkar
A1 Shubha Tole
A1 Richard M. Gronostajski
A1 Linda J. Richards
YR 2008
UL http://www.jneurosci.org/content/28/47/12328.abstract
AB The hippocampus plays an integral role in spatial navigation, learning and memory, and is a major site for adult neurogenesis. Critical to these functions is the proper organization of the hippocampus during development. Radial glia are known to regulate hippocampal formation, but their precise function in this process is yet to be defined. We find that in Nuclear Factor I b (Nfib)-deficient mice, a subpopulation of glia from the ammonic neuroepithelium of the hippocampus fail to develop. This results in severe morphological defects, including a failure of the hippocampal fissure, and subsequently the dentate gyrus, to form. As in wild-type mice, immature nestin-positive glia, which encompass all types of radial glia, populate the hippocampus in Nfib-deficient mice at embryonic day 15. However, these fail to mature into GLAST- and GFAP-positive glia, and the supragranular glial bundle is absent. In contrast, the fimbrial glial bundle forms, but alone is insufficient for proper hippocampal morphogenesis. Dentate granule neurons are present in the mutant hippocampus but their migration is aberrant, likely resulting from the lack of the complete radial glial scaffold usually provided by both glial bundles. These data demonstrate a role for Nfib in hippocampal fissure and dentate gyrus formation, and that distinct glial bundles are critical for correct hippocampal morphogenesis.