TY - JOUR T1 - Which GABA<sub>A</sub> Receptor Subunits Are Necessary for Tonic Inhibition in the Hippocampus? JF - The Journal of Neuroscience JO - J. Neurosci. SP - 1421 LP - 1426 DO - 10.1523/JNEUROSCI.4751-07.2008 VL - 28 IS - 6 AU - Joseph Glykys AU - Edward O. Mann AU - Istvan Mody Y1 - 2008/02/06 UR - http://www.jneurosci.org/content/28/6/1421.abstract N2 - GABAA receptors (GABAARs) assembled of different subunits mediate tonic and phasic inhibition in hippocampal neurons. CA1/CA3 pyramidal cells (PCs) predominantly express α5 subunits whereas dentate gyrus granule cells (DGGCs) and molecular layer (ML) interneurons predominantly express δ subunits. Both α5- and δ-containing GABAARs mediate tonic inhibition. We have shown previously that mice lacking α5 subunits (Gabra5−/−) have a residual tonic current in CA1/CA3 PCs because of an upregulation of δ subunits, but the basis of the residual tonic current in DGGCs and ML interneurons of mice lacking the δ subunit (Gabrd−/−) is still unknown. We now show that wild-type DGGCs have a small tonic current mediated by α5 subunit-containing GABAARs responsible for ∼29% of the total tonic current. To better identify the GABAARs mediating tonic inhibition in hippocampal neurons, we generated mice lacking both α5 and δ subunits (Gabra5/Gabrd−/−). Recordings from CA1/CA3 PCs, DGGCs, and ML interneurons in these mice show an absence of tonic currents without compensatory changes in spontaneous IPSCs (sIPSCs), sEPSCs, and membrane resistance. The absence of tonic inhibition results in spontaneous gamma oscillations recordable in vitro in the CA3 pyramidal layer of these mice, which can be mimicked in wild-type mice by blocking α5 subunit-containing GABAARs with 50 nm L-655,708. In conclusion, depending on the cell type, the α5 and δ subunits are the principal GABAAR subunits responsible for mediating the lion's share of tonic inhibition in hippocampal neurons. ER -