RT Journal Article SR Electronic T1 Dendrites of Mammalian Neurons Contain Specialized P-Body-Like Structures That Respond to Neuronal Activation JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 13793 OP 13804 DO 10.1523/JNEUROSCI.4155-08.2008 VO 28 IS 51 A1 Nicolas Cougot A1 Suvendra N. Bhattacharyya A1 Lucie Tapia-Arancibia A1 Remy Bordonné A1 Witold Filipowicz A1 Edouard Bertrand A1 Florence Rage YR 2008 UL http://www.jneurosci.org/content/28/51/13793.abstract AB Intracellular mRNA transport and local translation play a key role in neuronal physiology. Translationally repressed mRNAs are transported as a part of ribonucleoprotein (RNP) particles to distant dendritic sites, but the properties of different RNP particles and mechanisms of their repression and transport remain largely unknown. Here, we describe a new class of RNP-particles, the dendritic P-body-like structures (dlPbodies), which are present in the soma and dendrites of mammalian neurons and have both similarities and differences to P-bodies of non-neuronal cells. These structures stain positively for a number of P-body and microRNP components, a microRNA-repressed mRNA and some translational repressors. They appear more heterogeneous than P-bodies of HeLa cells, and they rarely contain the exonuclease Xrn1 but are positive for rRNA. These particles show motorized movements along dendrites and relocalize to distant sites in response to synaptic activation. Furthermore, Dcp1a is stably associated with dlP-bodies in unstimulated cells, but exchanges rapidly on neuronal activation, concomitantly with the loss of Ago2 from dlP-bodies. Thus, dlP-bodies may regulate local translation by storing repressed mRNPs in unstimulated cells, and releasing them on synaptic activation.