PT  - JOURNAL ARTICLE
AU  - Yong Ho Kim
AU  - Chul-Kyu Park
AU  - Seung Keun Back
AU  - C. Justin Lee
AU  - Se Jin Hwang
AU  - Yong Chul Bae
AU  - Heung Sik Na
AU  - Joong Soo Kim
AU  - Sung Jun Jung
AU  - Seog Bae Oh
TI  - Membrane-Delimited Coupling of TRPV1 and mGluR5 on Presynaptic Terminals of Nociceptive Neurons
AID  - 10.1523/JNEUROSCI.5030-08.2009
DP  - 2009 Aug 12
TA  - The Journal of Neuroscience
PG  - 10000--10009
VI  - 29
IP  - 32
4099  - http://www.jneurosci.org/content/29/32/10000.short
4100  - http://www.jneurosci.org/content/29/32/10000.full
SO  - J. Neurosci.2009 Aug 12; 29
AB  - Transient receptor potential vanilloid subtype 1 (TRPV1) and metabotropic glutamate receptor 5 (mGluR5) located on peripheral sensory terminals have been shown to play critical roles in the transduction and modulation of pain sensation. To date, however, very little is known regarding the significance of functional expression of mGluR5 and TRPV1 on the central terminals of sensory neurons in the dorsal horn of the spinal cord. Here we show that TRPV1 on central presynaptic terminals is coupled to mGluR5 in a membrane-delimited manner, thereby contributing to the modulation of nociceptive synaptic transmission in the substantia gelatinosa neurons of the spinal cord. Further, our results demonstrate that TRPV1 is involved in the pain behaviors induced by spinal mGluR5 activation, and diacylglycerol produced by the activation of mGluR5 mediates functional coupling of mGluR5 and TRPV1 on the presynaptic terminals. Thus, mGluR5–TRPV1 coupling on the central presynaptic terminals of nociceptive neurons may be an important mechanism underlying central sensitization under pathological pain conditions.