PT  - JOURNAL ARTICLE
AU  - Xuekun Li
AU  - Xiaobing Tang
AU  - Beata Jablonska
AU  - Adan Aguirre
AU  - Vittorio Gallo
AU  - Marla B. Luskin
TI  - p27<sup>KIP1</sup> Regulates Neurogenesis in the Rostral Migratory Stream and Olfactory Bulb of the Postnatal Mouse
AID  - 10.1523/JNEUROSCI.4051-08.2009
DP  - 2009 Mar 04
TA  - The Journal of Neuroscience
PG  - 2902--2914
VI  - 29
IP  - 9
4099  - http://www.jneurosci.org/content/29/9/2902.short
4100  - http://www.jneurosci.org/content/29/9/2902.full
SO  - J. Neurosci.2009 Mar 04; 29
AB  - Neuronal progenitor cells of the anterior subventricular zone (SVZa) migrate along the rostral migratory stream (RMS) to the olfactory bulb, where they exit the cell cycle and differentiate. The molecular mechanisms that regulate SVZa progenitor proliferation and cell-cycle exit are largely undefined. We investigated the role of p27KIP1 in regulating cell proliferation and survival in the RMS and olfactory bulb between postnatal day 1 (P1) and P14, the peak period of olfactory bulb neuron generation. A large proportion of cells in the RMS and the olfactory bulb express cytoplasmic p27KIP1, but a small percentage display high nuclear p27KIP1 immunostaining, which exhibit a caudallow–rostralhigh gradient: lowest in the SVZa and highest in the glomerular layer of the olfactory bulb. p27KIP1 is also present in the nucleus and/or the cytoplasm of neuron-specific type III β-tubulin(+) cells. Cells with strong nuclear p27KIP1 expression are BrdU(−) and Ki67(−). The percentage of BrdU(+) cells in the SVZa, RMS, and olfactory bulb is higher in p27KIP1 null than wild-type (WT) mice at all ages analyzed. Consistent with these findings, p27KIP1 overexpression in cultured p27KIP1 null and WT SVZ cells reduced cell proliferation and self-renewal. Finally, in p27KIP1 null mice, the diameter of the horizontal limb of the RMS is larger than in WT mice, and development of the olfactory bulb granule cell layer is delayed, together with increased apoptotic cell density. Our results indicate that in the postnatal brain, p27KIP1 regulates the proliferation and survival of neuronal cells in the RMS and olfactory bulb.