RT Journal Article SR Electronic T1 Drosophila Larvae Establish Appetitive Olfactory Memories via Mushroom Body Neurons of Embryonic Origin JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 10655 OP 10666 DO 10.1523/JNEUROSCI.1281-10.2010 VO 30 IS 32 A1 Dennis Pauls A1 Mareike Selcho A1 Nanae Gendre A1 Reinhard F. Stocker A1 Andreas S. Thum YR 2010 UL http://www.jneurosci.org/content/30/32/10655.abstract AB Insect mushroom bodies are required for diverse behavioral functions, including odor learning and memory. Using the numerically simple olfactory pathway of the Drosophila melanogaster larva, we provide evidence that the formation of appetitive olfactory associations relies on embryonic-born intrinsic mushroom body neurons (Kenyon cells). The participation of larval-born Kenyon cells, i.e., neurons that become gradually integrated in the developing mushroom body during larval life, in this task is unlikely. These data provide important insights into how a small set of identified Kenyon cells can store and integrate olfactory information in a developing brain. To investigate possible functional subdivisions of the larval mushroom body, we anatomically disentangle its input and output neurons at the single-cell level. Based on this approach, we define 10 subdomains of the larval mushroom body that may be implicated in mediating specific interactions between the olfactory pathway, modulatory neurons, and neuronal output.