PT  - JOURNAL ARTICLE
AU  - Attila I. Gulyás
AU  - Gergely G. Szabó
AU  - István Ulbert
AU  - Noémi Holderith
AU  - Hannah Monyer
AU  - Ferenc Erdélyi
AU  - Gábor Szabó
AU  - Tamás F. Freund
AU  - Norbert Hájos
TI  - Parvalbumin-Containing Fast-Spiking Basket Cells Generate the Field Potential Oscillations Induced by Cholinergic Receptor Activation in the Hippocampus
AID  - 10.1523/JNEUROSCI.4104-10.2010
DP  - 2010 Nov 10
TA  - The Journal of Neuroscience
PG  - 15134--15145
VI  - 30
IP  - 45
4099  - http://www.jneurosci.org/content/30/45/15134.short
4100  - http://www.jneurosci.org/content/30/45/15134.full
SO  - J. Neurosci.2010 Nov 10; 30
AB  - Gamma frequency oscillations in cortical regions can be recorded during cognitive processes, including attention or memory tasks. These oscillations are generated locally as a result of reciprocal interactions between excitatory pyramidal cells and perisomatic inhibitory interneurons. Here, we examined the contribution of the three perisomatic interneuron types—the parvalbumin-containing fast-spiking basket cells (FSBCs) and axo-axonic cells (AACs), as well as the cholecystokinin-containing regular-spiking basket cells (RSBCs) to cholinergically induced oscillations in hippocampal slices, a rhythmic activity that captures several features of the gamma oscillations recorded in vivo. By analyzing the spiking activities of single neurons recorded in parallel with local field potentials, we found that all three cell types fired phase locked to the carbachol-induced oscillations, although with different frequencies and precision. During these oscillations, FSBCs fired the most with the highest accuracy compared with the discharge of AACs and RSBCs. In further experiments, we showed that activation of μ-opioid receptors by DAMGO ([D-Ala2,N-Me-Phe4,Gly5-ol]enkephalin acetate), which significantly reduced the inhibitory, but not excitatory, transmission, suppressed or even blocked network oscillations both in vitro and in vivo, leading to the desynchronization of pyramidal cell firing. Using paired recordings, we demonstrated that carbachol application blocked GABA release from RSBCs and reduced it from FSBCs and AACs, whereas DAMGO further suppressed the GABA release only from FSBCs, but not from AACs. These results collectively suggest that the rhythmic perisomatic inhibition, generating oscillatory fluctuation in local field potentials after carbachol treatment of hippocampal slices, is the result of periodic GABA release from FSBCs.