PT  - JOURNAL ARTICLE
AU  - Mark P. Beenhakker
AU  - John R. Huguenard
TI  - Astrocytes as Gatekeepers of GABA<sub>B</sub> Receptor Function
AID  - 10.1523/JNEUROSCI.3243-10.2010
DP  - 2010 Nov 10
TA  - The Journal of Neuroscience
PG  - 15262--15276
VI  - 30
IP  - 45
4099  - http://www.jneurosci.org/content/30/45/15262.short
4100  - http://www.jneurosci.org/content/30/45/15262.full
SO  - J. Neurosci.2010 Nov 10; 30
AB  - The long-lasting actions of the inhibitory neurotransmitter GABA result from the activation of metabotropic GABAB receptors. Enhanced GABAB-mediated IPSCs are critical for the generation of generalized thalamocortical seizures. Here, we demonstrate that GABAB-mediated IPSCs recorded in the thalamus are primarily defined by GABA diffusion and activation of distal extrasynaptic receptors potentially up to tens of micrometers from synapses. We also show that this diffusion is differentially regulated by two astrocytic GABA transporters, GAT1 and GAT3, which are localized near and far from synapses, respectively. A biologically constrained model of GABA diffusion and uptake shows how the two GATs differentially modulate amplitude and duration of GABAB IPSCs. Specifically, the perisynaptic expression of GAT1 enables it to regulate GABA levels near synapses and selectively modulate peak IPSC amplitude, which is primarily dependent on perisynaptic receptor occupancy. GAT3 expression, however, is broader and includes distal extrasynaptic regions. As such, GAT3 acts as a gatekeeper to prevent diffusion of GABA away from synapses toward extrasynaptic regions that contain a potentially enormous pool of GABAB receptors. Targeting this gatekeeper function may provide new pharmacotherapeutic opportunities to prevent the excessive GABAB receptor activation that appears necessary for thalamic seizure generation.