TY - JOUR T1 - The <em>Caenorhabditis elegans</em> JIP3 Protein UNC-16 Functions As an Adaptor to Link Kinesin-1 with Cytoplasmic Dynein JF - The Journal of Neuroscience JO - J. Neurosci. SP - 2216 LP - 2224 DO - 10.1523/JNEUROSCI.2653-10.2011 VL - 31 IS - 6 AU - Makoto Arimoto AU - Sandhya P. Koushika AU - Bikash C. Choudhary AU - Chris Li AU - Kunihiro Matsumoto AU - Naoki Hisamoto Y1 - 2011/02/09 UR - http://www.jneurosci.org/content/31/6/2216.abstract N2 - Kinesin-1 is a microtubule plus-end-directed motor that transports various cargos along the axon. Previous studies have elucidated the physical and genetic interactions between kinesin-1 and cytoplasmic dynein, a microtubule minus-end-directed motor, in neuronal cells. However, the physiological importance of kinesin-1 in the dynein-dependent retrograde transport of cargo molecules remains obscure. Here, we show that Caenorhabditis elegans kinesin-1 forms a complex with dynein via its interaction with UNC-16, which binds to the dynein light intermediate (DLI) chain. Both kinesin-1 and UNC-16 are required for localization of DLI-1 at the plus ends of nerve process microtubules. In addition, retrograde transport of APL-1 depends on kinesin-1, UNC-16, and dynein. These results suggest that kinesin-1 mediates the anterograde transport of dynein using UNC-16 as a scaffold and that dynein in turn mediates the retrograde transport of cargo molecules in vivo. Thus, UNC-16 functions as an adaptor for kinesin-1-mediated transport of dynein. ER -