PT  - JOURNAL ARTICLE
AU  - Eric S. Sweet
AU  - Michelle L. Previtera
AU  - Jose R. Fernández
AU  - Erik I. Charych
AU  - Chia-Yi Tseng
AU  - Munjin Kwon
AU  - Valentin Starovoytov
AU  - James Q. Zheng
AU  - Bonnie L. Firestein
TI  - PSD-95 Alters Microtubule Dynamics via an Association With EB3
AID  - 10.1523/JNEUROSCI.1205-10.2011
DP  - 2011 Jan 19
TA  - The Journal of Neuroscience
PG  - 1038--1047
VI  - 31
IP  - 3
4099  - http://www.jneurosci.org/content/31/3/1038.short
4100  - http://www.jneurosci.org/content/31/3/1038.full
SO  - J. Neurosci.2011 Jan 19; 31
AB  - Little is known about how the neuronal cytoskeleton is regulated when a dendrite decides whether to branch or not. Previously, we reported that postsynaptic density protein 95 (PSD-95) acts as a stop signal for dendrite branching. It is yet to be elucidated how PSD-95 affects the cytoskeleton and how this regulation relates to the dendritic arbor. Here, we show that the SH3 (src homology 3) domain of PSD-95 interacts with a proline-rich region within the microtubule end-binding protein EB3. Overexpression of PSD-95 or mutant EB3 results in a decreased lifetime of EB3 comets in dendrites. In line with these data, transfected rat neurons show that overexpression of PSD-95 results in less organized microtubules at dendritic branch points and decreased dendritogensis. The interaction between PSD-95 and EB3 elucidates a function for a novel region of EB3 and provides a new and important mechanism for the regulation of microtubules in determining dendritic morphology.