TY - JOUR T1 - The role of spinal cord cyclic AMP in the acoustic startle response in rats JF - The Journal of Neuroscience JO - J. Neurosci. SP - 3250 LP - 3257 DO - 10.1523/JNEUROSCI.06-11-03250.1986 VL - 6 IS - 11 AU - JH Kehne AU - DI Astrachan AU - E Astrachan AU - JF Tallman AU - M Davis Y1 - 1986/11/01 UR - http://www.jneurosci.org/content/6/11/3250.abstract N2 - Drugs thought to increase intracellular levels of cAMP were infused intrathecally into the subarachnoid space of the lumbar spinal cord, and the effects on the acoustic startle response in rats were measured. Intrathecal infusions of the cAMP analogs dibutyryl cAMP or 8-bromo cAMP (12.5–100 micrograms) produced marked, dose-dependent increases in startle amplitude compared to the infusion of artificial cerebrospinal fluid (CSF). Local infusions of dibutyryl cAMP at more rostral levels of the spinal cord or brain failed to mimic the excitatory effect seen following lumbar intrathecal infusion. No excitation of startle was seen following intrathecal infusion of cAMP itself, ATP, 5′-AMP, or dibutyryl cGMP. A weak excitation of startle was seen following intrathecal, but not intraventricular, infusion of the water-soluble adenylate cyclase activator forskolin 7-deacetyl-7–O-hemisuccinic acid (forskolin-DHA; 5.0–100 micrograms, in artificial CSF), whereas forskolin itself [0.01–200 micrograms, in dimethyl sulfoxide (DMSO)] was without consistent effect. Finally, intrathecal infusion of the selective phosphodiesterase inhibitor Rolipram (12.5–200 micrograms) produced a marked excitation of startle similar in magnitude to the effects produced by cAMP analogs. The excitatory effects of intrathecally infused dibutyryl cAMP, 8-bromo cAMP, forskolin-DHA, or Rolipram support a functional link between spinal cord cAMP and the acoustic startle reflex. Possible sites of cAMP action on startle are discussed. ER -