RT Journal Article
SR Electronic
T1 Biological activities of nerve growth factor bound to nitrocellulose paper by Western blotting
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 3624
OP 3632
DO 10.1523/JNEUROSCI.08-10-03624.1988
VO 8
IS 10
A1 B Pettmann
A1 M Manthorpe
A1 JA Powell
A1 S Varon
YR 1988
UL http://www.jneurosci.org/content/8/10/3624.abstract
AB We have previously developed a cell-blot technique to visualize directly in tissue extracts molecules that display the biological activity of ciliary neuronotrophic factors (CNTFs). This technique involves SDS-PAGE of the tissue extract, Western blotting on nitrocellulose paper, neuronal cell culture on the paper, and, using a vital dye, visualization of the neurons that selectively survive on the trophic factor band. In this report, we show that (1) NGF, either purified or in a crude extract from submaxillary glands, can also be successfully recognized using a slightly modified cell-blot technique; (2) a variety of ganglionic neurons can respond to distinct nitrocellulose-anchored trophic factors; (3) while CNTF and NGF can both support the survival of their common target cells, only NGF also promotes neuritic extension; and (4) both the dimeric and the monomeric forms of immobilized beta-NGF are active.