TY - JOUR T1 - Heterogeneity of GABAergic cells in cat visual cortex JF - The Journal of Neuroscience JO - J. Neurosci. SP - 988 LP - 1000 DO - 10.1523/JNEUROSCI.08-03-00988.1988 VL - 8 IS - 3 AU - H Demeulemeester AU - F Vandesande AU - GA Orban AU - C Brandon AU - JJ Vanderhaeghen Y1 - 1988/03/01 UR - http://www.jneurosci.org/content/8/3/988.abstract N2 - Antibodies against neuropeptides and against a vitamin D-dependent calcium-binding protein (CaBP) label small cells with nonpyramidal-like morphology in the cat visual cortex (areas 17, 18, and 19). Since GABAergic cells are interneurons, a double-staining procedure was used to test for the coexistence of cholecystokinin (CCK), somatostatin (SRIF), neuropeptide Y (NPY), corticotropin-releasing factor (CRF), vasoactive intestinal polypeptide (VIP), and CaBP with glutamic acid decarboxylase (GAD). Our results show that CRF and VIP do not coexist with GAD, while the 3 other peptides and CaBP do. Hence GAD-positive cells can be subdivided into 4 broad groups: (1) cells that are only GAD-positive, (2) cells that are GAD- and CaBP-positive, (3) GAD- positive neurons also containing CCK, and (4) GAD-positive cells that also contain SRIF. A small subset of class 2 also contains SRIF and most cells of class 4 also contain NPY. The 4 classes of GAD-positive cells differ in laminar position: class 1 predominates in layers IV and V, classes 2 and 3 in the upper laminae (II and III), and class 4 in the deepest layer (VI). ER -