TY - JOUR T1 - Nuclear Factor κB-Dependent Neurite Remodeling Is Mediated by Notch Pathway JF - The Journal of Neuroscience JO - J. Neurosci. SP - 11697 LP - 11705 DO - 10.1523/JNEUROSCI.1113-11.2011 VL - 31 IS - 32 AU - Sara Anna Bonini AU - Giulia Ferrari-Toninelli AU - Daniela Uberti AU - Mery Montinaro AU - Laura Buizza AU - Cristina Lanni AU - Mariagrazia Grilli AU - Maurizio Memo Y1 - 2011/08/10 UR - http://www.jneurosci.org/content/31/32/11697.abstract N2 - In this study, we evaluated whether a cross talk between nuclear factor κB (NF-κB) and Notch may take place and contribute to regulate cell morphology and/or neuronal network in primary cortical neurons. We found that lack of p50, either induced acutely by inhibiting p50 nuclear translocation or genetically in p50−/− mice, results in cortical neurons characterized by reduced neurite branching, loss of varicosities, and Notch1 signaling hyperactivation. The neuronal morphological effects found in p50−/− cortical cells were reversed after treatment with the γ-secretase inhibitor DAPT (N-[N-(3,5-difluorophenacetyl)-1-alanyl 1]-S-phenylglycine t-butyl ester) or Notch RNA interference. Together, these data suggested that morphological abnormalities in p50−/− cortical neurons were dependent on Notch pathway hyperactivation, with Notch ligand Jagged1 being a major player in mediating such effect. In this line, we demonstrated that the p50 subunit acts as transcriptional repressor of Jagged1. We also found altered distribution of Notch1 and Jagged1 immunoreactivity in the cortex of p50−/− mice compared with wild-type littermates at postnatal day 1. These data suggest the relevance of future studies on the role of Notch/NF-κB cross talk in regulating cortex structural plasticity in physiological and pathological conditions. ER -