RT Journal Article
SR Electronic
T1 Differential localization of microtubules in cerebellar cells
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 1961
OP 1967
DO 10.1523/JNEUROSCI.09-06-01961.1989
VO 9
IS 6
A1 I Ginzburg
A1 L Behar
A1 UZ Littauer
A1 WS Griffin
YR 1989
UL http://www.jneurosci.org/content/9/6/1961.abstract
AB The distribution and subcellular localization of microtubules in rat brain cerebellum was analyzed by immunohistochemistry with antibodies prepared against 3 synthetic peptides corresponding to the C-terminal region of beta-tubulin. The peptides used correspond to amino acid positions 416–425 (peptide 1), 416–431 (peptide 2), and 426–445 (peptide 4). The antibodies thus obtained displayed a remarkable specificity in reacting with different cell types in the rat cerebellum. Antibodies directed against peptide 1 primarily stained Purkinje cells and their dendrites and axons. Peptide 2 antibodies preferentially stained the glomeruli, while antibodies directed against peptide 4 preferentially stained Bergmann glial fibers. These results are discussed in terms of dissimilarities in microtubule organization and masking of epitopes by microtubule-associated proteins (MAPs) in individual cerebellar cells, which may be related to specific functional properties.