TY - JOUR T1 - Gβ<sub>3</sub> Is Required for Normal Light ON Responses and Synaptic Maintenance JF - The Journal of Neuroscience JO - J. Neurosci. SP - 11343 LP - 11355 DO - 10.1523/JNEUROSCI.1436-12.2012 VL - 32 IS - 33 AU - Anuradha Dhingra AU - Hariharasubramanian Ramakrishnan AU - Adam Neinstein AU - Marie E. Fina AU - Ying Xu AU - Jian Li AU - Daniel C. Chung AU - Arkady Lyubarsky AU - Noga Vardi Y1 - 2012/08/15 UR - http://www.jneurosci.org/content/32/33/11343.abstract N2 - Heterotrimeric G-proteins, comprising Gα and Gβγ subunits, couple metabotropic receptors to various downstream effectors and contribute to assembling and trafficking receptor-based signaling complexes. A G-protein β subunit, Gβ3, plays a critical role in several physiological processes, as a polymorphism in its gene is associated with a risk factor for several disorders. Retinal ON bipolar cells express Gβ3, and they provide an excellent system to study its role. In the ON bipolar cells, mGluR6 inverts the photoreceptor's signal via a cascade in which glutamate released from photoreceptors closes the TRPM1 channel. This cascade is essential for vision since deficiencies in its proteins lead to complete congenital stationary night blindness. Here we report that Gβ3 participates in the G-protein heterotrimer that couples mGluR6 to TRPM1. Gβ3 deletion in mouse greatly reduces the light response under both scotopic and photopic conditions, but it does not eliminate it. In addition, Gβ3 deletion causes mislocalization and downregulation of most cascade elements and modulators. Furthermore, Gβ3 may play a role in synaptic maintenance since in its absence, the number of invaginating rod bipolar dendrites is greatly reduced, a deficit that was not observed at 3 weeks, the end of the developmental period. ER -