TY - JOUR T1 - The Role of Arp2/3 in Growth Cone Actin Dynamics and Guidance Is Substrate Dependent JF - The Journal of Neuroscience JO - J. Neurosci. SP - 5895 LP - 5908 DO - 10.1523/JNEUROSCI.0672-14.2014 VL - 34 IS - 17 AU - José E. San Miguel-Ruiz AU - Paul C. Letourneau Y1 - 2014/04/23 UR - http://www.jneurosci.org/content/34/17/5895.abstract N2 - During development extrinsic guidance cues modulate the peripheral actin network in growth cones to direct axons to their targets. We wanted to understand the role of the actin nucleator Arp2/3 in growth cone actin dynamics and guidance. Since growth cones migrate in association with diverse adhesive substrates during development, we probed the hypothesis that the functional significance of Arp2/3 is substrate dependent. We report that Arp2/3 inhibition led to a reduction in the number of filopodia and growth cone F-actin content on laminin and L1. However, we found substrate-dependent differences in growth cone motility, actin retrograde flow, and guidance after Arp2/3 inhibition, suggesting that its role, and perhaps that of other actin binding proteins, in growth cone motility is substrate dependent. ER -