PT  - JOURNAL ARTICLE
AU  - Qinghua Fang
AU  - Ying Zhao
AU  - Adam Drew Herbst
AU  - Brian N. Kim
AU  - Manfred Lindau
TI  - Positively Charged Amino Acids at the SNAP-25 C Terminus Determine Fusion Rates, Fusion Pore Properties, and Energetics of Tight SNARE Complex Zippering
AID  - 10.1523/JNEUROSCI.2905-14.2015
DP  - 2015 Feb 18
TA  - The Journal of Neuroscience
PG  - 3230--3239
VI  - 35
IP  - 7
4099  - http://www.jneurosci.org/content/35/7/3230.short
4100  - http://www.jneurosci.org/content/35/7/3230.full
SO  - J. Neurosci.2015 Feb 18; 35
AB  - SNAP-25 is a Q-SNARE protein mediating exocytosis of neurosecretory vesicles including chromaffin granules. Previous results with a SNAP-25 construct lacking the nine C terminal residues (SNAP-25Δ9) showed changed fusion pore properties (Fang et al., 2008), suggesting a model for fusion pore mechanics that couple C terminal zipping of the SNARE complex to the opening of the fusion pore. The deleted fragment contains the positively charged residues R198 and K201, adjacent to layers 7 and 8 of the SNARE complex. To determine how fusion pore conductance and dynamics depend on these residues, single exocytotic events in bovine chromaffin cells expressing R198Q, R198E, K201Q, or K201E mutants were investigated by carbon fiber amperometry and cell-attached patch capacitance measurements. Coarse grain molecular dynamics simulations revealed spontaneous transitions between a loose and tightly zippered state at the SNARE complex C terminus. The SNAP-25 K201Q mutant showed no changes compared with SNAP-25 wild-type. However, K201E, R198Q, and R198E displayed reduced release frequencies, slower release kinetics, and prolonged fusion pore duration that were correlated with reduced probability to engage in the tightly zippered state. The results show that the positively charged amino acids at the SNAP-25 C terminus promote tight SNARE complex zippering and are required for high release frequency and rapid release in individual fusion events.