RT Journal Article SR Electronic T1 The Subventricular Zone Continues to Generate Corpus Callosum and Rostral Migratory Stream Astroglia in Normal Adult Mice JF The Journal of Neuroscience JO J. Neurosci. FD Society for Neuroscience SP 3756 OP 3763 DO 10.1523/JNEUROSCI.3454-14.2015 VO 35 IS 9 A1 Jiho Sohn A1 Lori Orosco A1 Fuzheng Guo A1 Seung-Hyuk Chung A1 Peter Bannerman A1 Emily Mills Ko A1 Kostas Zarbalis A1 Wenbin Deng A1 David Pleasure YR 2015 UL http://www.jneurosci.org/content/35/9/3756.abstract AB Astrocytes are the most abundant cells in the CNS, and have many essential functions, including maintenance of blood–brain barrier integrity, and CNS water, ion, and glutamate homeostasis. Mammalian astrogliogenesis has generally been considered to be completed soon after birth, and to be reactivated in later life only under pathological circumstances. Here, by using genetic fate-mapping, we demonstrate that new corpus callosum astrocytes are continuously generated from nestin+ subventricular zone (SVZ) neural progenitor cells (NPCs) in normal adult mice. These nestin fate-mapped corpus callosum astrocytes are uniformly postmitotic, express glutamate receptors, and form aquaporin-4+ perivascular endfeet. The entry of new astrocytes from the SVZ into the corpus callosum appears to be balanced by astroglial apoptosis, because overall numbers of corpus callosum astrocytes remain constant during normal adulthood. Nestin fate-mapped astrocytes also flow anteriorly from the SVZ in association with the rostral migratory stream, but do not penetrate into the deeper layers of the olfactory bulb. Production of new astrocytes from nestin+ NPCs is absent in the normal adult cortex, striatum, and spinal cord. Our study is the first to demonstrate ongoing SVZ astrogliogenesis in the normal adult mammalian forebrain.