TY - JOUR T1 - Amyloid-β Impairs Synaptic Inhibition via GABA<sub>A</sub> Receptor Endocytosis JF - The Journal of Neuroscience JO - J. Neurosci. SP - 9205 LP - 9210 DO - 10.1523/JNEUROSCI.0950-15.2015 VL - 35 IS - 24 AU - Daniel Ulrich Y1 - 2015/06/17 UR - http://www.jneurosci.org/content/35/24/9205.abstract N2 - Amyloid β (Aβ) is thought to play an important role in the pathogenesis of Alzheimer's disease. Aβ may exert its neurotoxic effects via multiple mechanisms and in particular through degradation of excitatory synaptic transmission associated with impaired synaptic plasticity. In contrast, much less is known about Aβ effects at inhibitory synapses. This study investigates the impact of acute Aβ1-42 application on GABAergic synaptic transmission in rat somatosensory cortex in vitro. Whole-cell voltage-clamp recordings were obtained from layer V pyramidal cells, and monosynaptic GABAA receptor-mediated IPSCs were elicited. Bath-applied Aβ (1 μm) depressed the IPSCs on average to 60% of control, whereas a reversed sequence control peptide was ineffective. Paired-pulse stimuli indicated a postsynaptic site of action. This was further corroborated by a decreased postsynaptic responsiveness to local puffs of the GABAA receptor agonist isoguvacine. The Aβ-induced IPSC decline could be prevented with intracellular applications of p4, a blocker of GABAA receptor internalization. It is concluded that Aβ weakens synaptic inhibition via downregulation of GABAA receptors. ER -