TY - JOUR T1 - Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish JF - The Journal of Neuroscience JO - J. Neurosci. SP - 15291 LP - 15294 DO - 10.1523/JNEUROSCI.3050-15.2015 VL - 35 IS - 46 AU - Chun-feng Shang AU - Xiao-quan Li AU - Chen Yin AU - Bing Liu AU - Yu-fan Wang AU - Zhuan Zhou AU - Jiu-lin Du Y1 - 2015/11/18 UR - http://www.jneurosci.org/content/35/46/15291.abstract N2 - Dopamine plays crucial roles in a broad spectrum of brain functions, and neural circuit mechanisms underlying dopaminergic regulation have been intensively studied in the past decade. As larval zebrafish have relatively simple and highly conserved dopaminergic systems, it can serve as an ideal vertebrate animal model to tackle this issue at a whole-brain scale. For this purpose, it is important to develop methods for monitoring endogenous dopamine release in intact larval zebrafish. Here, we developed a real-time method to monitor dopamine release at high spatiotemporal resolution in the brain of awake larval zebrafish using carbon fiber microelectrodes. As an example for application, we combined this method with genetic tools and in vivo calcium imaging and found that food extract can activate pretectal dopaminergic neurons, which in turn release dopamine at the visual center through their projection, providing a dopaminergic circuit mechanism for olfactory modulation of visual functions. Thus, our study demonstrates, for the first time, the utility of carbon fiber microelectrodes for monitoring sensory-evoked dopamine release in the brain of an awake small organism.SIGNIFICANCE STATEMENT With carbon fiber microelectrodes, we have succeeded in monitoring sensory-evoked dopamine release in the brain of an awake small organism for the first time. By elucidating the circuitry origin of the dopamine release, we illustrated the potential application of this method in dissection of the neural circuitry mechanisms underlying dopaminergic neuromodulation. ER -