RT Journal Article
SR Electronic
T1 Selective phosphorylation of AMPA receptor contributes to the network of long-term potentiation in the anterior cingulate cortex
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 0925-17
DO 10.1523/JNEUROSCI.0925-17.2017
A1 Qian Song
A1 Hong-Wei Zheng
A1 Xu-Hui Li
A1 Richard L. Huganir
A1 Thomas Kuner
A1 Min Zhuo
A1 Tao Chen
YR 2017
UL http://www.jneurosci.org/content/early/2017/08/01/JNEUROSCI.0925-17.2017.abstract
AB Phosphorylation of AMPA receptor GluA1 plays important roles in synaptic potentiation. Most previous studies have been carried out in the hippocampus, while the roles of GluA1 phosphorylation in the cortex remain unknown. Here we investigated the involvement of phosphorylation of GluA1 in the LTP in the anterior cingulate cortex (ACC) using mice with GluA1 knock-in mutation at the PKA phosphorylation site serine 845 (s845A) or CaMKII/PKC phosphorylation site serine 831 (s831A). The network LTP which is constructed by multiple recording of LTP at different location within the ACC was also investigated. We found that the expression of LTP and network LTP was significantly impaired in the s845A but not in the s831A mice. By contrast, basal synaptic transmission and NMDA receptor mediated responses were not affected. Furthermore, to uncover potential information under the current acquired data, a new method for reconstruction and better visualization of the signals was developed to observe the spatial localizations and dynamic temporal changes of fEPSP signals and multiple LTP responses within the ACC circuit. Our results provide strong evidence that PKA phosphorylation of the GluA1 is important for the network LTP expression in the ACC.SIGNIFICANCE STATEMENTPrevious studies have shown that PKA and PKC phosphorylation of AMPA receptor GluA1 play critical roles in LTP in the hippocampus, while the roles of GluA1 phosphorylation in the cortex remain unknown. In the present study, by combining a 64-channel multi-electrode (MED64) system and a novel analysis and visualization method, we observed the accurate spatial localization and dynamic temporal changes of network fEPSP signals and LTP responses within the ACC circuit and found that PKA but not PKC phosphorylation of the GluA1 is required for the LTP in the ACC.