RT Journal Article
SR Electronic
T1 New observations in neuroscience using superresolution microscopy
JF The Journal of Neuroscience
JO J. Neurosci.
FD Society for Neuroscience
SP 9459
OP 9467
DO 10.1523/JNEUROSCI.1678-18.2018
VO 38
IS 44
A1 Michihiro Igarashi
A1 Motohiro Nozumi
A1 Ling-Gang Wu
A1 Francesca Cella Zanacchi
A1 István Katona
A1 László Barna
A1 Pingyong Xu
A1 Mingshu Zhang
A1 Fudong Xue
A1 Edward Boyden
YR 2018
UL http://www.jneurosci.org/content/38/44/9459.abstract
AB Superresolution microscopy (SM) techniques are among the revolutionary methods for molecular and cellular observations in the 21st century. SM techniques overcome optical limitations, and several new observations using SM lead us to expect these techniques to have a large impact on neuroscience in the near future. Several types of SM have been developed, including structured illumination microscopy (SIM), stimulated emission depletion microscopy (STED), and photoactivated localization microscopy (PALM)/stochastic optical reconstruction microscopy (STORM), each with special features. In this Minisymposium, experts in these different types of SM discuss the new structural and functional information about specific important molecules in neuroscience that has been gained with SM. Using these techniques, we have revealed novel mechanisms of endocytosis in nerve growth, fusion pore dynamics, and described quantitative new properties of excitatory and inhibitory synapses. Additional powerful techniques, including single molecule-guided Bayesian localization SM (SIMBA) and expansion microscopy (ExM), alone or combined with super-resolution observation, are also introduced in this session.