Frequency of neurons expressing VGluT2 mRNA containing or lacking TH immunoreactivity in the PBP or the PNa
| Percentage of VGluT2-only neurons or VGluT2-TH neurons within the total population of VGluT2 mRNA-expressing neurons | Percentage of TH-only neurons or VGluT2-TH neurons within the total population of TH-IR neurons | |||
|---|---|---|---|---|
| VGluT2-only neurons | VGluT2-TH neurons | TH-only neurons | VGluT2-TH neurons | |
| PBP mediolateral distance from the RLib | ||||
| 0–200 μm | ||||
| Subject 1 | 78.8% (n = 53) | 21.2% (n = 13) | 44.2% (n = 10) | 55.8% (n = 13) |
| Subject 2 | 65.5% (n = 55) | 34.5% (n = 29) | 40.7% (n = 20) | 59.3% (n = 29) |
| Subject 3 | 72.7% (n = 40) | 27.3% (n = 15) | 11.8% (n = 2) | 88.2% (n = 15) |
| Mean ± SEM | 72.3 ± 3.2% (n = 148) | 27.7 ± 3.2% (n = 57) | 36.3 ± 6.4% (n = 32) | 63.6 ± 6.4% (n = 57) |
| 200–400 μm | ||||
| Subject 1 | 82.4% (n = 41) | 17.6% (n = 8) | 42.9% (n = 6) | 57.1% (n = 8) |
| Subject 2 | 70.6% (n = 36) | 29.4% (n = 14) | 34.1% (n = 7) | 65.9% (n = 14) |
| Subject 3 | 90.4% (n = 47) | 9.6% (n = 5) | 37.5% (n = 3) | 62.5% (n = 5) |
| Mean ± SEM | 79.3 ± 5.4% (n = 124) | 20.7 ± 5.4% (n = 27) | 38.3 ± 2.2% (n = 16) | 61.7 ± 2.2% (n = 27) |
| PN mediolateral distance from the IFc | ||||
| 0–200 μm | ||||
| Subject 1 | 74.6% (n = 47) | 25.4% (n = 16) | 47.9% (n = 15) | 52.1% (n = 16) |
| Subject 2 | 72.7% (n = 8) | 27.3% (n = 3) | 76.9% (n = 10) | 23.1% (n = 3) |
| Subject 3 | 80.0% (n = 12) | 20.0% (n = 3) | 40.0% (n = 2) | 60.0% (n = 3) |
| Mean ± SEM | 75.5 ± 1.5% (n = 67) | 24.5 ± 1.5% (n = 22) | 53.2 ± 8.3% (n = 27) | 46.8 ± 8.3% (n = 22) |
↵aVGluT2-only neurons (expressing VGluT2 mRNA but lacking TH immunoreactivity), VGluT2-TH neurons (coexpressing VGluT2 mRNA and TH immunoreactivity), or TH-only neurons (containing TH immunoreactivity but lacking VGluT2 mRNA) were counted in every 16th 5-μm-thick coronal section from three different rats (subjects 1–3) at the level of −5.28 mm from bregma. n, Number of counted neurons within each segment from a total of three sections, with each section from a different rat.
↵bNeurons were counted from the medial (border between the rostral linear nucleus, RLi, and the PBP) to the lateral aspects of the PBP within two segments (0–200 and 200–400 μm).
↵cNeurons were counted from the medial (border between the interfascicular nucleus, IF, and the PN) to the lateral aspects of the PN within a 0–200 μm segment.